This study was undertaken to find out main toxic components in venom of Agkistrodon b. iyJiuvicaudus and to prepare antiserum to venom. Through the experiment, venom of A. b. brevicaudus was fractionated into 7 fractions through DEAF-Sephadex A-50´ and observation were made with toxic reaction of fractioned venom of A. b. brevicaudus, neutralizing effect of anlasenun for highly toxic fraction and finally, comparison of neutralizing effect of antisera for frac-*nated venom and original crude venom.
The results ara summarized as follows:
-1. Fractionation of A. b. lrrrevicaudus venom by DEAE sephadex A-50 column chromatography
:resulted in seven peaks. The amount of total protein were the highest in 4th fraction.
2~ To investigate the toxicity of each fraction, hemorrhagic activity of each fraction were corn-"-,.,Pared. As a result, most strong hemorrhagic activity was shown in 6th fraction. Then 4th, 7th 44s-fraction were in that order.
´11, :Neutralizing effect of rat: antisera for 2nd, 4th and 6th fraction were compared. Most effective neutralizing(antilethal) activity was shown in antiserum for 4th fraction and neutralizing effect ´of antiserum for 4th fraction was similar to that of crude venom.
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